Clinical Study on Anti Microbial effect of Charme Acidic Water on Bacteria

Bacteria	Count prior to testing	5 sec	10 sec	30 sec
E-Coli	3.94 mil	<100	<100	<100
Straphylococcus aureaus	12.5 mil	<100	<100	<100
Streptococcus pyogenes	8.95 mi	<100	<100	<100
Pseudomonas aeruginosa	17.82 mil	<100	<100	<100
MRSA	11.24 mi	<100	<100	<100
Salmonella	13.80 mil	<100	<100	<100
Sporular bacteria	11.40 mi	7,500	3,100	<100

Testing agency: Food & Drug Safety Center, Japan
Bacterial broth: Low pH acidic water = 1 : 100

Sterilization comparison of conventional disinfectant products

Disinfectant	General bacteria	Pseudomonas aeruginosa	MSRA	Tubercule bacillus	Eumycetes	Spore
Low pH Acidic water	E	E	E	E	E	E
Glutaraldehyde	E	E	E	E	E	E
Formaldehyde	E	E	E	E	E	L
Sodium hyochlorite	E	E	E	L	E	L
Ethanol for disinfection	E	E	E	E	E	N
Iodoform	E	E	E	E	E	L
Saponated cresol solution	E	E	E	E	L	N
Alkyldimethylbenzyl ammonium chloride	E	E	E	N	L	N
Chlorhexidine gluconate	E	E	E	N	L	N
Ampholytic surface active agent	E	E	E	L	L	N

E: Effective
L: Less Effective
N: Not Effective

	Low pH Acidic Water	Tap Water	Conventional Disinfectant	Chlorinated Bleaching agent
pH	0-2.8	6.2-8.6	2.4-5.5	9-11
Residual Chlorine Concentrate	1-10 ppm	0-2 ppm	10-50 ppm	500-600 ppm
Inpurity (metal)	None	Some	Some	Large
Skin Irratation	Few	Few	Some	Large
Chlorine Odor	Few	Few	Some	Large

Safety Test

	Test material	Test animal	Evaluation
Acute oral toxicity test	Low pH acidic water	Mouse	No issue
Eye irritation test	Low pH acidic water	Rabbit	No issue
Primary skin irritation test	Low pH acidic water	Rabbit	No issue

In one Japanese study, more than 22,000 patients with eczema were treated with low pH water.
Researchers found that 85% of the patients improved with NO SIDE EFFECTS.

The Journal of Cosmetic Dermatology, Feb 2004 Issue Volume 17 - #2 (The efficacy intolerability of electrolyzed oxidized water in treating mild to moderate acne), tested Charme on people with acne.
After just a few weeks, there was a 41% reduction in acne on the side of the face treated with Charme.
There was a 76% reduction in blackheads and 42% reduction in whiteheads. 80% of the participants rated Charme as good or excellent and 75% preferred it to topical medications.

In January 2002, the California Skin Research Institute did a study that showed that showed how effective Revenir was on facials lines and wrinkles. 80% of the people showed signficant improvement in the the smoothing of facial lines, 77% showed less fine lines and wrinkles and a 326% improvement in the distance between facial lines. All within 3 months!

The California Skin Research also studies hyperpigementation and reported an 89% improvement.